A serious bug affecting representative subsets of original-style sequences in ASTRAL 1.57 and 1.59 has now been fixed. All original- style subsets filtered by E-value and percent identity contained extra domains. Genetic domain subsets, the default sets in 1.57 and 1.59, were not affected by the bug. The affected subsets have been regenerated correctly, and are now available on our website through the normal download mechanisms. Files containing the subsets which were originally released are still available through direct access to ASTRAL files, or by downloading a tarball of all files; however, these files have been clearly identified by prepending the word "broken" to their names. Thanks to John Spalding for reporting this bug. (Fixed 9/10/02) The sequences based on ATOM records for four domains are truncated in ASTRAL 1.59. The entries for these domains in the RAF file were corrupted, leading to the bug. The RAF file has been corrected, and the ATOM sequences will be fixed in the next release. These are the four domains affected: d1hcja_ d1hcjb_ d1hcjc_ d1hcjd_ There are some bugs in the underlying PDB files which we do not attempt to fix; we have identified files with missing residues in the SEQRES records (which were present in the ATOM records), and cases where the residues in the SEQRES and ATOM fields do not match. These are lists of the domains affected: Domains with missing SEQRES records (cases where coordinates for a residue are given in the ATOM records, but the residue is not included in the SEQRES records): d1fcs__ d1ifa__ d1mdc__ d9pti__ Domains with mismatches between SEQRES and ATOM records (cases where a coordinates for a residue are given in the ATOM records, but the SEQRES records indicate a different residue should appear at that position. d2fam__ k 146 -> a d4ccp__ d 272 -> n d1fna__ r 6 -> g d2plv1_ l 7 -> s d1s01__ p 56 -> n d2bce__ n 187 -> q d4icd__ e 331 -> q d8gpb__ k 840 -> a d3gbp__ q 25 -> e d1pda_2 a 241 -> g d2pf2_2 r 15 -> e d1hra__ p 1 -> m